L1CAM ELISA 试剂盒
Quick Overview for L1CAM ELISA 试剂盒 (ABIN2703287)
抗原
See all L1CAM ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.8 pg/mL
-
原理
- Human L1CAM (NCAM-L1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair recognizes human L1CAM. Other species not yet determined.
-
灵敏度
- 0.8 pg/mL
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
L1 Cell Adhesion Molecule (L1CAM) ELISA Kit
L1CAM 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
L1 Cell Adhesion Molecule (L1CAM) ELISA KitL1CAM 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
L1 Cell Adhesion Molecule (L1CAM) ELISA KitL1CAM 适用: 大鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
-
-
应用备注
- Recommended Dilution for serum and plasma samples5,000,000 fold
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
样品制备
-
For example, add 2 μL of serum or plasma into a tube with 198 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 198 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample. Mix through and then pipette 2 μL of prepared 10,000-fold diluted sample into a tube with 998 μL 1x Assay Diluent to prepare a final 5,000,000 fold diluted sample.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
- L1CAM (L1 Cell Adhesion Molecule (L1CAM))
-
别名
- L1CAM
-
背景
-
Gene Names: L1CAM CAML1 MIC5
Protein names: Neural cell adhesion molecule L1 (N-CAM-L1) (NCAM-L1) (CD antigen CD171)
-
基因ID
- 3897
-
UniProt
- P32004
-
途径
- Synaptic Membrane
抗原 See all L1CAM ELISA试剂盒
-
